Intermediate

Part:BBa_K368005:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-20)


PhiC31o Integrase + SV40 Polyadenylation site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2042
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 475
    Illegal PstI site found at 1012
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 859
    Illegal AgeI site found at 1154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 201
    Illegal BsaI.rc site found at 538
    Illegal BsaI.rc site found at 781
    Illegal BsaI.rc site found at 941
    Illegal BsaI.rc site found at 957
    Illegal BsaI.rc site found at 1294
    Illegal SapI.rc site found at 444
    Illegal SapI.rc site found at 1356


Design Notes

add a promoter and express PhiC31o in eucaryotic cells


Source

bought from addgene; Raymond CS et al: High-Efficiency FLP and PhiC31 Site-Specific Recombination in Mammalian Cells (2007) SV40PA is from BBa_K368018

References